R8758

Sigma

 

RPMI-1640 Medium

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, cell culture tested

MDL number:MFCD00217820

Description

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ApplicationRPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy's 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
LinkageNeed FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Properties

sterilitysterile-filtered
formliquid
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.2-8°C

Safety

WGK Germany3

Related Products

Related productF2442, Fetal Bovine Serum

References

referenceMoore GE, et al., Culture of normal human leukocytes J. Am. Med. Assoc. 199, 519-524, (1967)
 Mizrahi A, and Moore GE., Partial substitution of serum in hematopoietic cell line media by synthetic polymers Appl. Microbiol. 19, 906-910, (1970) Abstract